THE REACTION WITH RED CELLS OF 7S RABBIT ANTIBODY, ITS SUB-UNITS AND THEIR RECOMBINANTS

Abstract

Univalent 3.5S antibody fragments obtained from 7S rabbit antibody specific for human group A red cells, have a very much smaller association constant in their reaction with human group A₁ red cells than have 7S or 5S antibody. Univalent 5S recombinants have a similarly low association constant, whereas bivalent 5S recombinants have an association constant similar to that of 7S antibody.

It is concluded that this supports the authors' hypothesis that rabbit anti-A 7S antibody is normally attached to red cells by both its specific antibody groups.