LIPIDS OF DEBARYOMYCES HANSENII

Abstract

Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Edward M. Devine, Jr. Lipids of Debaryomyces hansenii. J. Bacteriol. 89:1488-1493. 1965.-The separation of neutral lipids and phospholipids from the lipid extract of Debaryomyces hansenii NRRL Y-1448 was accomplished by using a single column packed with silicic acid. The neutral lipids comprised 67%, and the phospholipids 33%, of the total lipid extract. Qualitative and quantitative characterization of the lipids was effected by various analytical procedures. Gas chromatography of the fatty acid methyl esters showed 11 acids, of which 59.7% were unsaturated. The most abundant among the unsaturated acids was the monounsaturated C18 acid (50.1%). Among the saturated acids, palmitic acid (23.5%) prevailed. Ratios of fatty acid to glycerol, phosphorus to glycerol, and phosphorus to fatty acid were ascertained for combined cuts from the neutral lipids and the phospholipids. The presence of ergosterol, stigmasterol, and an unidentified sterol was established by gas chromatography. Also present were saturated hydrocarbons containing 16 to 39 carbon atoms, C22 being the most prevalent.