Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1965 May;89(5):1314-21.
doi: 10.1128/jb.89.5.1314-1321.1965.

UNSTABLE LINKAGE BETWEEN GENETIC MARKERS IN TRANSFORMATION

M S KELLY, R H PRITCHARD

UNSTABLE LINKAGE BETWEEN GENETIC MARKERS IN TRANSFORMATION

M S KELLY et al. J Bacteriol. 1965 May.

Abstract

Kelly, M. (Hammersmith Hospital, London, England), and R. H. Pritchard. Unstable linkage between genetic markers in transformation. J. Bacteriol. 89:1314-1321. 1965.-A new type of association of genetic markers in a transformation system has been discovered. Linkage between certain markers is detectable only under particular conditions of deoxyribonucleic acid (DNA) extraction and is sensitive to dilution of the DNA. The data suggest that this unstable association occurs between markers which are on the same molecule but separated by a molecular distance sufficiently great for linkage to be preserved only by mild DNA extraction procedures and at high concentrations of DNA. Linkage can be stabilized by dilution in the presence of a carrier DNA. By use of these unstable linkages, the genetic map of Bacillus subtilis has been extended in both directions from the aromatic gene cluster.

PubMed Disclaimer

References

    1. Biochem J. 1957 Jul;66(3):495-504 - PubMed
    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. Nature. 1959 Dec 5;184:1780-2 - PubMed
    1. Proc Natl Acad Sci U S A. 1963 Jun;49:806-13 - PubMed
    1. Biochem J. 1956 Feb;62(2):315-23 - PubMed

LinkOut - more resources