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Comparative Study
. 1992 Nov;174(22):7458-62.
doi: 10.1128/jb.174.22.7458-7462.1992.

Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli

M M Faraldo  1 , M A de Pedro, J Berenguer
Affiliations
Comparative Study

Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli

M M Faraldo et al. J Bacteriol. 1992 Nov.

Abstract

The nucleotide sequence of the slpA gene, which is responsible for the synthesis of the S-layer protein of Thermus thermophilus HB8, is described. This gene is transcribed as a unit in which the coding region is preceded by a 127-base-long leader mRNA sequence. The promoter region is also recognized by the RNA polymerase of Escherichia coli because of the presence of homologous -35 and -10 boxes. Homologies with other promoters from Thermus spp. are also presented.

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