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. 1992 Oct 2;581(1):93-9.
doi: 10.1016/0378-4347(92)80451-u.

Determination of vinorelbine (Navelbine) in tumour cells by high-performance liquid chromatography

V Debal  1 , H Morjani, J M Millot, J F Angiboust, B Gourdier, M Manfait
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Determination of vinorelbine (Navelbine) in tumour cells by high-performance liquid chromatography

V Debal et al. J Chromatogr. .

Abstract

A high-performance liquid chromatographic method has been developed for the determination within tumour cells of a new vinca alkaloid, vinorelbine. Extractions of vinorelbine from cells were carried out using absolute ethanol. The extracts were injected into a reversed-phase system consisting of two Novapak C18 columns connected in series. The mobile phase was acetonitrile-phosphate buffer, pH 2.7 (60:40, v/v). Using a fluorescence detection, the limit of determination was 8 pmol injected. This method would be suitable for studying the cellular pharmacokinetics of vinorelbine in patients.

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