Seasonal changes in the activation of crossbridge motions of isolated thick filament from Limulus striated muscle

Abstract

In dynamic light scattering, measurements of the intensity-intensity time correlation function from a suspension of rod-like particles of length L could reveal dynamical information related to translational and internal motions of those particles. For a suspension of thick filaments isolated from the myosin-regulated, striated muscles of Limulus at KL greater than 1 (where K is the scattering vector), the average characteristic linewidth (gamma) increased with the addition of Ca2+ or with the depletion of ATP. The increase in the gamma with the addition of Ca2+ could be due to the presence of energy-requiring, high-frequency motions of the crossbridges activated by Ca2+. The increase in gamma which occurred with the depletion of ATP was assumed to be mainly due to the thermal motions of the crossbridges after they had moved radially away from the filament backbone. The percentage increase in gamma following the addition of Ca2+ was found to be seasonal, i.e., values of gamma obtained from thick filaments isolated between the middle of June and the middle of September were smaller than those obtained during the rest of the year. The effect of temperature on the percentage increase in gamma was also different. The increase showed a maximum at about 35 degrees C during the summer and at about 25 degrees C at other times. However, the percentage increase in gamma developed under ATP-depleted conditions showed no temperature-related maximum. The number of bound Ca2+ per myosin molecule was 1 during the summer and 2 at other times.