[Effect of peptidoglycan preparations on phage inactivation: decrease in phage 187 binding using monosaccharides]

Abstract

Monosaccharides were found to be capable to decreasing the binding of phage 187 with peptidoglycan. Galactosamine hydrochloride and glucosamine hydrochloride proved to be the most effective, followed in order of decreasing activity by N-acetylgalactosamine, N-acetylglucosamine, glucose and galactose; mannose produced a weak effect. Ca2+ ions increased the reduction of phage peptiglycan binding with N-acetylgalactosamine and N-acetylglucosamine. Both of these monosaccharides also reduced the adsorption of phage 187 by the Staphylococcus aureus cells. In the medium enriched with Ca2+ ions; in the absence of Ca2+, they were, however, incapable of releasing the phage irreversibly bound with the peptidoglycan.