Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1965 Jul 1;122(1):173-80.
doi: 10.1084/jem.122.1.173.

FORMATION OF CHARCOT-LEYDEN CRYSTALS IN HUMAN EOSINOPHILS AND BASOPHILS AND STUDY OF THE COMPOSITION OF ISOLATED CRYSTALS

G T ARCHER, A BLACKWOOD

FORMATION OF CHARCOT-LEYDEN CRYSTALS IN HUMAN EOSINOPHILS AND BASOPHILS AND STUDY OF THE COMPOSITION OF ISOLATED CRYSTALS

G T ARCHER et al. J Exp Med. .

Abstract

Methods are described for the isolation of human eosinophils and basophils from donor blood. Using these cell preparations Charcot-Leyden crystals were found to originate from both eosinophils and basophils when the cells were suspended in hypotonic saline solution. The crystals formed also when saline extracts of eosinophils and basophils were concentrated by ultrafiltration through dialysis tubing. Fractions of eosinophils were prepared and the crystals were obtained from the cytoplasmic fraction but not from the nuclear or granular fractions. Chemical studies showed the crystals to be protein in nature and some evidence is presented which suggests that RNA may decrease the tendency for the protein to crystallize out of solution.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1951 Mar;6(4):562-7 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Anat Rec. 1953 May;116(1):53-74 - PubMed
    1. Biochem J. 1954 Apr;56(4):631-9 - PubMed
    1. J Exp Med. 1960 Dec 1;112:983-1004 - PubMed