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. 1965 Aug;90(2):312-5.
doi: 10.1128/jb.90.2.312-315.1965.

PURIFICATION OF A SALT-REQUIRING ENZYME FROM AN OBLIGATELY HALOPHILIC BACTERIUM

P K HOLMES, H O HALVORSON

PURIFICATION OF A SALT-REQUIRING ENZYME FROM AN OBLIGATELY HALOPHILIC BACTERIUM

P K HOLMES et al. J Bacteriol. 1965 Aug.

Abstract

Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson. Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312-315. 1965.-The discovery that some halophilic enzymes may be reactivated from the salt-free and inactive state has facilitated the purification of nicotinamide adenine dinucleotide-linked malic acid dehydrogenase from crude extracts of Halobacterium salinarium. In the absence of large amounts of salt, the enzyme was totally inactive; yet, in this salt-free state, it could be purified by conventional techniques. The highly purified halophilic enzyme was shown to require a high concentration of salt for activity.

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