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. 1965 Apr;95(1):161-6.
doi: 10.1042/bj0950161.

THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES

A L GREENBAUM, J B CLARK, P MCLEAN

THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES

A L GREENBAUM et al. Biochem J. 1965 Apr.

Abstract

1. A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0.02mug. of NAD(+) or NADH or 0.01mug. of NADP(+) or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature.

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