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. 1965 Jul;13(4):559-63.
doi: 10.1128/am.13.4.559-563.1965.

RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS

R S MARSHALL, J F STEENBERGEN, L S MCCLUNG

RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS

R S MARSHALL et al. Appl Microbiol. 1965 Jul.

Abstract

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

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References

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