[The effect of hyperthermia against pancreatic cancer cell--various examinations including flow cytometric bromodeoxyuridine/DNA analysis]

Abstract

We investigated the effect of hyperthermia against pancreatic cancer cell in various aspects including of cytokinetics. The hyperthermia above 43 degrees C was thought to have strong effect for the studies on surviving cell number and morphological change of Capan-2 and RWP-1 cells after heating treatment. The cell inoculated to the nude mice subcutaneously after heating treatment at 43 degrees C was recognized the tumor forming at 3 weeks later, however, the growth speed was slower than that of control to which untreated Capan-2 cells were inoculated. As the case of 44 degrees C, the tumor forming was not recognized at all. The cytokinetics was measured by flow cytometric bromodeoxyuridine (BrdUrd)/DNA analysis. Up to 43 degrees C in accordance with increase in the heating temperature, the accumulation in G2M phase increased, and the amount of BrdUrd incorporated 5 phase decreased. At the temperature above 44 degrees C, BrdUrd was hardly incorporated into S phase cells and the cell cycle was almost stopped. These results suggested the usefulness of hyperthermia and strong effect of heating treatment above 44 degrees C against pancreatic cancer cell.