In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV)
- PMID: 1441736
- PMCID: PMC7133906
- DOI: 10.1016/0168-1702(92)90144-x
In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV)
Abstract
Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera.
Similar articles
-
Subgenomic mRNA of Aura alphavirus is packaged into virions.J Virol. 1994 Jan;68(1):56-62. doi: 10.1128/JVI.68.1.56-62.1994. J Virol. 1994. PMID: 7902874 Free PMC article.
-
Synthesis in vitro of rabbit hemorrhagic disease virus subgenomic RNA by internal initiation on (-)sense genomic RNA: mapping of a subgenomic promoter.J Biol Chem. 2004 Apr 23;279(17):17013-8. doi: 10.1074/jbc.M313674200. Epub 2004 Jan 26. J Biol Chem. 2004. PMID: 14744857
-
Isolation and identification of a non-haemagglutinating strain of rabbit hemorrhagic disease virus from China and sequence analysis for the VP60 Gene.Virus Genes. 2007 Dec;35(3):745-52. doi: 10.1007/s11262-007-0155-3. Epub 2007 Aug 21. Virus Genes. 2007. PMID: 17705093
-
Haemorrhagic disease of lagomorphs: evidence for a calicivirus.Vet Microbiol. 1992 Nov;33(1-4):375-81. doi: 10.1016/0378-1135(92)90065-2. Vet Microbiol. 1992. PMID: 1282757 Review.
-
Identification of the viral haemorrhagic disease virus of rabbits as a calicivirus.Rev Sci Tech. 1991 Jun;10(2):311-23. doi: 10.20506/rst.10.2.548. Rev Sci Tech. 1991. PMID: 1662097 Review.
Cited by
-
A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV).Microorganisms. 2021 Apr 30;9(5):972. doi: 10.3390/microorganisms9050972. Microorganisms. 2021. PMID: 33946292 Free PMC article. Review.
-
Calicivirus RNA-Dependent RNA Polymerases: Evolution, Structure, Protein Dynamics, and Function.Front Microbiol. 2019 Jun 6;10:1280. doi: 10.3389/fmicb.2019.01280. eCollection 2019. Front Microbiol. 2019. PMID: 31244803 Free PMC article. Review.
-
First Detection and Circulation of RHDV2 in New Zealand.Viruses. 2024 Mar 28;16(4):519. doi: 10.3390/v16040519. Viruses. 2024. PMID: 38675862 Free PMC article.
-
A pandemic strain of calicivirus threatens rabbit industries in the Americas.Virol J. 2007 Oct 2;4:96. doi: 10.1186/1743-422X-4-96. Virol J. 2007. PMID: 17910765 Free PMC article.
-
European brown hare syndrome virus: relationship to rabbit hemorrhagic disease virus and other caliciviruses.J Virol. 1994 Aug;68(8):5164-73. doi: 10.1128/JVI.68.8.5164-5173.1994. J Virol. 1994. PMID: 7518531 Free PMC article.
References
-
- Argüello J.L., Llanos Pellitero A., Perez-Ordoyo Garcia L.I. Enfermedad vírica hemorrágica del conejo en España. Med. Vet. 1988;5:645–650.
-
- Black D.N., Burroughs J.N., Harris T.J.R., Brown F. The structure and replication of Calicivirus RNA. Nature. 1978;274:614–615. - PubMed
-
- Chirgwin J.A., Przybyla A.E., MacDonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979;18:5294–5299. - PubMed
-
- Davis L.G., Dibner M.D., Battey J.F. Elsevier; Amsterdam: 1986. pp. 143–146. (Basic Methods in Molecular Biology).
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources