Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Oct 15;287 ( Pt 2)(Pt 2):443-6.
doi: 10.1042/bj2870443.

Characterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes

O A Coso  1 , A Díaz Añel, H Martinetto, J P Muschietti, M Kazanietz, D Fraidenraich, H N Torres, M M Flawia
Affiliations

Characterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes

O A Coso et al. Biochem J. .

Abstract

A guanosine 5'-[gamma-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this blocking capacity. Incubation of T. cruzi membranes with activated pertussis toxin and [adenylate-32P]NAD+ led to the incorporation of radioactivity into a labelled product with an apparent M(r) of approx. 43,000. Crude membranes were electrophoresed on SDS/polyacrylamide gels and analysed, by Western blotting, with GA/1 anti-alpha common, AS/7 anti-alpha t, anti-alpha i1 and anti-alpha i2 polyclonal antibodies. These procedures led to the identification of a specific polypeptide band of about 43 kDa. Another polypeptide reacting with the SW/1 anti-beta antibody, of about 30 kDa, was also detected in the membrane fraction.

PubMed Disclaimer

References

    1. J Cyclic Nucleotide Res. 1976 Nov-Dec;2(6):381-92 - PubMed
    1. Pflugers Arch. 1986 Sep;407(3):264-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7809-13 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Jan;83(2):265-9 - PubMed
    1. Biochem J. 1987 Sep 1;246(2):431-9 - PubMed

Publication types

MeSH terms