doi: 10.1111/j.1432-1033.1977.tb11884.x.
Adenosine triphosphatase activity associated with purified cholinergic synaptic vesicles of Torpedo marmorata
- PMID: 144598
- DOI: 10.1111/j.1432-1033.1977.tb11884.x
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Adenosine triphosphatase activity associated with purified cholinergic synaptic vesicles of Torpedo marmorata
Eur J Biochem.
.
Free article
Abstract
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromagography on CPG-10-3000 porous glass beads. The synaptic vesicles have a buoyant density of 1.057 g/ml. The purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+, K+-stimulated ATPase activity. They contain a ouabaininsensitive, Na+, K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline.
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