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. 1962 Apr;83(4):914-8.
doi: 10.1128/jb.83.4.914-918.1962.

Purification of staphylococcal alpha-hemolysin

M A MADOFF, L WEINSTEIN

Purification of staphylococcal alpha-hemolysin

M A MADOFF et al. J Bacteriol. 1962 Apr.

Abstract

Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914-918. 1962. - Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.

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References

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