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. 1992 Dec;174(23):7827-30.
doi: 10.1128/jb.174.23.7827-7830.1992.

Escherichia coli B lacks one of the two initiator tRNA species present in E. coli K-12

N Mandal  1 , U L RajBhandary
Affiliations

Escherichia coli B lacks one of the two initiator tRNA species present in E. coli K-12

N Mandal et al. J Bacteriol. 1992 Dec.

Abstract

We show that the metY locus which specifies tRNA(2fMet) in Escherichia coli K-12 specifies tRNA(1fMet) in E. coli B. This conclusion is based on results of Southern blot analysis of E. coli B and K-12 DNAs and on polymerase chain reaction amplification, cloning, and sequencing of an approximately 200-bp region of DNA corresponding to the metY loci of E. coli B and E. coli K-12. We also show that the metY locus of E. coli B is transcriptionally active. E. coli strains transformed with the multicopy plasmid vector pUC19 carrying the metY locus of E. coli B overproduce tRNA(1fMet) in E. coli B and E. coli K-12 in contrast to strains transformed with pUC19 carrying the corresponding locus from E. coli K-12, which overproduce tRNA(2fMet).

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