Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1962 Feb;12(2):385-410.
doi: 10.1083/jcb.12.2.385.

Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide

S L PALAY, S M McGEE-RUSSELL, S GORDON Jr, M A GRILLO

Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide

S L PALAY et al. J Cell Biol. 1962 Feb.

Abstract

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.

PubMed Disclaimer

References

    1. J Biophys Biochem Cytol. 1961 Feb;9:409-14 - PubMed
    1. J Biophys Biochem Cytol. 1960 Jul;7:685-96 - PubMed
    1. Z Zellforsch Mikrosk Anat. 1956;44(3):263-81 - PubMed
    1. J Biophys Biochem Cytol. 1961 Apr;9:853-77 - PubMed
    1. Pharmacol Rev. 1958 Jun;10(2):165-273 - PubMed