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. 1977 Oct;4(10):3483-96.
doi: 10.1093/nar/4.10.3483.

Studies on the biological role of DNA methylation: III Role in excision of one-genome long single-stranded phi X 174 DNA

J Friedman, A Friedmann, A Razin
Free PMC article

Studies on the biological role of DNA methylation: III Role in excision of one-genome long single-stranded phi X 174 DNA

J Friedman et al. Nucleic Acids Res. 1977 Oct.
Free PMC article

Abstract

Accumulation of replicative intermediates of the bacteriophage phi X174 was observed in E. coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron microscopy revealed that these molecules are composed of double-stranded DNA rings with multiple-genome length single-stranded "tails". These results suggest that the single 5-methylcytosine residue present in the phage DNA serves as a recognition site for the gene A protein mediating the excision of one-genome long phage DNA. This excision process is oligatory for the final maturation of the phage.

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References

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