doi: 10.1073/pnas.74.10.4306.
Oligomycin-dependent ionophoric protein subunit of mitochondrial adenosinetriphosphatase
- PMID: 144916
- PMCID: PMC431929
- DOI: 10.1073/pnas.74.10.4306
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Oligomycin-dependent ionophoric protein subunit of mitochondrial adenosinetriphosphatase
Proc Natl Acad Sci U S A.
1977 Oct.
Abstract
A proteolipid isolated from yeast mitochondrial adenosinetriphosphatase (subunit 9) (ATP phosphohydrolase; EC 3.6.1.3) by chloroform/methanol extraction has been shown to discharge photo-induced potentials across a planar phospholipid membrane containing bacteriorhodopsin. Oligomycin, a specific inhibitor of oxidative phosphorylation which binds to this protein, allows the potential gradient to be reestablished. When proteolipid was isolated from an oligomycin-resistant strain, ionophoric activity was still obtained but the effect was not reversed by oligomycin. These studies suggest that the hydrophobic subunit-9 polypeptide is the ionophoric component linking ATP synthesis (hydrolysis) with proton translocation.
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