Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine

Abstract

Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier studies it was shown that mice hyperimmunized with live vaccine of Salmonella enteritidis resisted intravenous challenge with 1,000 MLD of virulent strain 116-54 of S. enteritidis. Survivors of this challenge completely resisted additional intravenous challenge with 10,000 MLD of the same organism. Mononuclear phagocytes obtained from the abdominal cavity of mice immunized with live vaccine of S. enteritidis inhibited intracellular multiplication of virulent strain 116-54, regardless of the presence of antibody in the cell-culture medium. In the present study, mononuclear phagocytes were obtained in a nearly pure state from liver or subcutaneous tissue of mice and were maintained in vitro in good condition. These cells also resisted cellular degeneration caused by intracellular existence of virulent strain 116-54, regardless of the presence of antibody in the cell-culture medium. In contrast, cells obtained from normal mice or mice immunized with dead vaccine were subject to degeneration.