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. 2003:236:287-94.
doi: 10.1385/1-59259-413-1:287.

Virus-induced gene silencing

S P Dinesh-Kumar  1 , Radhamani Anandalakshmi, Rajendra Marathe, Michael Schiff, Yule Liu
Affiliations

Virus-induced gene silencing

S P Dinesh-Kumar et al. Methods Mol Biol. 2003.

Abstract

In the postgenomic era, large-scale functional genomic approaches are necessary for converting sequence information into functional information. A para-genetic approach, called virus-induced gene silencing (VIGS), offers a rapid means of gaining insight into gene function in plants. VIGS system could be used to suppress endogenous gene expression by infecting plants with a recombinant virus vector (VIGS vector) carrying host-derived sequence. Here, we describe the use of tobacco rattle virus (TRV)-based VIGS technique to study gene function in Nicotiana benthamiana and tomato.

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