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. 2003 Oct;47(10):3332-5.
doi: 10.1128/AAC.47.10.3332-3335.2003.

Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance

Antonio Doménech-Sánchez  1 , Luis Martínez-Martínez, Santiago Hernández-Allés, María del Carmen Conejo, Alvaro Pascual, Juan M Tomás, Sebastián Albertí, Vicente Javier Benedí
Affiliations

Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance

Antonio Doménech-Sánchez et al. Antimicrob Agents Chemother. 2003 Oct.

Abstract

OmpK35 from Klebsiella pneumoniae is the homologue of Escherichia coli OmpF porin. Expression of OmpK35 in K. pneumoniae strain CSUB10R (lacking both OmpK35 and OmpK36) decreased the MICs of cephalosporins and meropenem > or = 128-fold and decreased the MICs of imipenem, ciprofloxacin, and chloramphenicol > or = 8-fold. MIC reductions by OmpK35 were 4 times (cefepime), 8 times (cefotetan, cefotaxime, and cefpirome), or 128 times (ceftazidime) higher than those caused by OmpK36, but the MICs were similar or 1 dilution lower for other evaluated agents.

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Figures

FIG. 1.
FIG. 1.
Comparison by alignment of the deduced OmpK35 sequence from K. pneumoniae with the sequences of OmpF and OmpC from E. coli and OmpK36 from K. pneumoniae available in GenBank, EMBL, and DDBJ. Secondary structure motifs are described on the basis of the crystal structure of OmpK36. The numbering is based on the mature OmpK36. Conserved amino acids (shaded and boxed) and gaps introduced to maximize alignment (hyphens) are indicated.
FIG. 2.
FIG. 2.
Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of OMPs from K. pneumoniae strain CSUB10R (lane 1) and clones derived from CSUB10R carrying plasmids pSHA25K (lane 3) and pSHA16K (lane 4). K. pneumoniae isolate CSUB10S and its clone carrying pSHA16K are also shown in lanes 2 and 5, respectively. Porins expressed by each strain are indicated above each lane. The positions of LamB (white arrowhead) and OmpA (black arrowhead) homologues of K. pneumoniae are indicated to the left of lane 1.
See this image and copyright information in PMC

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