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. 2003 Aug;47(2):84-6.
doi: 10.1007/s00284-002-3939-7.

Random amplified polymorphic DNA profiles as a tool for the identification of Acanthamoeba divionensis

Antonio Ortega-Rivas  1 , Jacob Lorenzo-Morales, Violeta Alonso, Néstor J Abreu, Pilar Foronda, Antonio del Castillo, Basilio Valladares
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Random amplified polymorphic DNA profiles as a tool for the identification of Acanthamoeba divionensis

Antonio Ortega-Rivas et al. Curr Microbiol. 2003 Aug.

Abstract

In the present study, we demonstrated the Random Amplified Polymorphism DNA (RAPD) diagnostic validity. In our study, we have analyzed RAPD profiles searching for characteristic and useful bands for Acanthamoeba diagnosis at the species level. We found a distinctive 370-bp band in A. divionensis RAPD patterns, using the OPC14 primer (TGCGTGCTTG) (Operon Technologies, Inc., Alameda, CA). The band specificity was confirmed by hybridization, using it as a probe, against all OPC14 amplifications from 10 different Acanthamoeba species. Once we sequenced this band, we used it to design a specific primer pair which showed positive amplification only in A. divionensis isolates.

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