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. 2004 Jan;108(2):343-8.
doi: 10.1007/s00122-003-1434-6. Epub 2003 Sep 25.

Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags

J Wardrop  1 , J Fuller, W Powell, G C Machray
Affiliations

Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags

J Wardrop et al. Theor Appl Genet. 2004 Jan.

Abstract

Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

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