Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 Dec 15;376(Pt 3):807-12.
doi: 10.1042/BJ20031419.

Evidence for the transport of glutathione through ryanodine receptor channel type 1

Gábor Bánhegyi  1 , Miklós Csala, Gábor Nagy, Vincenzo Sorrentino, Rosella Fulceri, Angelo Benedetti
Affiliations
Comparative Study

Evidence for the transport of glutathione through ryanodine receptor channel type 1

Gábor Bánhegyi et al. Biochem J. .

Abstract

In the present study, we have investigated the role of RyR1 (ryanodine receptor calcium channel type 1) in glutathione (GSH) transport through the sarcoplasmic reticulum (SR) membrane of skeletal muscles. Lanthanum chloride, a prototypic blocker of cation channels, inhibited the influx and efflux of GSH in SR vesicles. Using a rapid-filtration-based assay and lanthanum chloride as a transport blocker, an uptake of radiolabelled GSH into SR vesicles was observed. Pretreatment of SR vesicles with the RyR1 antagonists Ruthenium Red and ryanodine as well as with lanthanum chloride blocked the GSH uptake. An SR-like GSH uptake appeared in microsomes obtained from an HEK-293 (human embryonic kidney 293) cell line after transfection of RyR1. These observations strongly suggest that RyR1 mediates GSH transport through the SR membranes of skeletal muscles.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1999 Oct 22;264(2):409-12 - PubMed
    1. J Biol Chem. 1999 Apr 30;274(18):12213-6 - PubMed
    1. Ann N Y Acad Sci. 1998 Sep 16;853:130-48 - PubMed
    1. J Biol Chem. 2000 Nov 24;275(47):36556-61 - PubMed
    1. Trends Biochem Sci. 2000 Nov;25(11):527-9 - PubMed

Publication types

MeSH terms