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Comparative Study
. 1992 Oct;83(10):1063-72.
doi: 10.1111/j.1349-7006.1992.tb02723.x.

Altered glycosylation of membrane glycoproteins associated with human mammary carcinoma

S Hiraizumi  1 , S Takasaki, N Ohuchi, Y Harada, M Nose, S Mori, A Kobata
Affiliations
Comparative Study

Altered glycosylation of membrane glycoproteins associated with human mammary carcinoma

S Hiraizumi et al. Jpn J Cancer Res. 1992 Oct.

Abstract

N-Linked sugar chains of normal mammary gland, mammary carcinomas (primary lesion), and axillary lymph node metastases of mammary carcinomas were released from their membrane preparations by hydrazinolysis and their structures were analyzed. Fractionation using a Datura stramonium agglutinin (DSA)-Sepharose column revealed that the metastasized carcinomas contain more than twice as much DSA-binding oligosaccharides as the normal gland, and the primary carcinomas contain an intermediate amount. These oligosaccharides were elucidated to have tri- and tetraantennary structures containing the GlcNAc beta 1-->6(GlcNAc beta 1-->2)Man group with and without N-acetyllactosamine repeating units. Lectin blot analysis of membrane glycoproteins and histochemical staining of tissues using biotinylated DSA indicated that these glycosylation changes predominantly occur in a limited number of glycoproteins with apparent molecular weights of 90, 160, and 210 kilodaltons, and mammary carcinomas are distinguishable from normal gland by their intense intracytoplasmic staining.

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References

    1. ) Yamashita , K. , Ohkura , T. , Tachibana , Y. , Takasaki , S. and Kobata , A.Comparative study of the oligosaccharides released from baby hamster kidney cells and their polyoma transformant by hydrazinolysis . J. Biol, Chem. , 259 , 10834 – 10840 ( 1984. ). - PubMed
    1. ) Pierce , M. and Arango , J.Rous sarcoma virus‐transformed baby hamster kidney cells express higher levels of asparamine‐linked tri‐and tetraantennary glycopeptides containing [GlcNAc‐β(1,6) Man‐α(1,6) Man] and poly‐N‐acetyllactosamine sequences than baby hamster kidney cells . J. Biol. Chem. , 261 , 10772 – 10777 ( 1986. ). - PubMed
    1. ) Hiraizumi , S. , Takasaki , S. , Shiroki , K. , Kochibe , N. and Kobata , A.Transfection with fragments of the adenovirus 12 gene induces tumorigenicity‐assiciated alteration of N‐linked sugar chains in rat cells . Arch. Biochem. Biophys. , 280 , 9 – 19 ( 1990. ). - PubMed
    1. ) Humphries , M. J. , Matsumoto , K. , White , S. L. , and Olden , K.Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16‐F10 murine melanoma cells . Proc. Natl. Acad. Sci. USA , 83 , 1752 – 1756 ( 1989. ). - PMC - PubMed
    1. ) Dennis , J. W. , Laferte , S. , Waghone , C. , Breitman , M. L. , and Kerbel , R.β1‐6 Branching of Asn‐linked oligosaccharides is directly associated with metastasis . Science. , 236 , 582 – 585 ( 1987. ). - PubMed

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