Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Oct;69(10):5914-8.
doi: 10.1128/AEM.69.10.5914-5918.2003.

PCR detection and analysis of the free-living amoeba Naegleria in hot springs in Yellowstone and Grand Teton National Parks

Kathy B Sheehan  1 , Jennifer A Fagg, Michael J Ferris, Joan M Henson
Affiliations

PCR detection and analysis of the free-living amoeba Naegleria in hot springs in Yellowstone and Grand Teton National Parks

Kathy B Sheehan et al. Appl Environ Microbiol. 2003 Oct.

Abstract

Free-living thermotolerant amoebae pose a significant health risk to people who soak and swim in habitats suitable for their growth, such as hot springs. In this survey of 23 different hot springs in Yellowstone and Grand Teton National Parks, we used PCR with primer sets specific for Naegleria to detect three sequence types that represent species not previously described, as well as a fourth sequence type identified as the pathogen Naegleria fowleri.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Neighbor-joining analysis using the 5.8S rRNA gene sequences and portions of the adjacent ITS-1 and ITS-2 regions of Naegleria species from GenBank and those PCR-amplified from bulk DNA extracted from hot springs in Yellowstone and Grand Teton National Parks. The tree was generated by using PAUP*, distances were calculated by using the Kimura two-parameter model, and bootstrap values of >50% are indicated. YNP, Yellowstone National Park.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Altschul, S. F., T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402. - PMC - PubMed
    1. Amaral Zettler, L. A., F. Gomez, E. Zettler, B. G. Keenan, R. Amils, and M. L. Sogin. 2002. Microbiology: eukaryotic diversity in Spain's River of Fire. Nature 417:137. - PubMed
    1. De Jonckheere, J. F. 2002. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41:309-342.
    1. Ferris, M. J., A. L. Ruff-Roberts, E. D. Kopczynski, M. M. Bateson, and D. M. Ward. 1996. Enrichment culture and microscopy conceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat. Appl. Environ. Microbiol. 62:1045-1050. - PMC - PubMed
    1. Jeanmougin, F., J. D. Thompson, M. Guoy, D. G. Higgins, and T. J. Gibson. 1998. Multiple sequence alignment with Clustal X. Trends Biochem. Sci. 23:403-405. - PubMed

Publication types

Associated data

LinkOut - more resources