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. 2003 Oct;69(10):6299-301.
doi: 10.1128/AEM.69.10.6299-6301.2003.

Development of a mariner-based transposon for use in Sorangium cellulosum

Bryan Julien  1 , Ruby Fehd
Affiliations

Development of a mariner-based transposon for use in Sorangium cellulosum

Bryan Julien et al. Appl Environ Microbiol. 2003 Oct.

Abstract

In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.

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Figures

FIG. 1.
FIG. 1.
Map of plasmid pKOS183-3. Raised arrowheads represent transcriptional start sites. Arrows on the circle designate the transposon inverted repeats.
FIG. 2.
FIG. 2.
Southern blot of transposon insertion strains. Lane 1, 1-kb ladder (the smallest band is 1.6 kb); lanes 2 to 10, nine independent transposon insertion strains.
See this image and copyright information in PMC

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