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. 2003 Oct;41(10):4812-4.
doi: 10.1128/JCM.41.10.4812-4814.2003.

Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis

Timothy J J Inglis  1 , Max Aravena-Roman, Simon Ching, Kevin Croft, V Wuthiekanun, Brian J Mee
Affiliations

Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis

Timothy J J Inglis et al. J Clin Microbiol. 2003 Oct.

Abstract

Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

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References

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