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Comparative Study
. 2003 Oct;140(4):647-52.
doi: 10.1038/sj.bjp.0705477.

Regulation of leptin distribution between plasma and cerebrospinal fluid by cholecystokinin receptors

Victoria Cano  1 , Laura Ezquerra, M Pilar Ramos, Mariano Ruiz-Gayo
Affiliations
Comparative Study

Regulation of leptin distribution between plasma and cerebrospinal fluid by cholecystokinin receptors

Victoria Cano et al. Br J Pharmacol. 2003 Oct.

Abstract

Cholecystokinin (CCK) is a postprandial hormone that elicits a satiating effect and regulates feeding behaviour. CCK has been shown to enhance the effect of leptin in several experimental paradigms. The goal of this work was to characterize the effect of endogenous CCK on plasma leptin content by using CCK receptor antagonists. Therefore, we administered SR-27897, a selective CCK1 receptor antagonist, and L-365260, a selective CCK2 receptor antagonist, to fed and food-deprived rats and determined plasma leptin concentration by enzyme immunoassay. Plasma insulin and glucose concentration as well as food intake were also determined. Under our conditions, SR-27897 increased plasma concentration of leptin both in fed and food-deprived rats. It also increased food intake as well as plasma concentration of insulin in fed animals. L-365260 increased plasma leptin concentration only in fed rats. In animals receiving exogenous leptin, CCK-8 increased the ratio between the concentration of leptin in cerebrospinal fluid and plasma. These results show that CCK receptor antagonists increases plasma concentration of leptin and suggest that endogenous CCK may facilitate the uptake of plasma leptin to the cerebrospinal fluid.

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Figures

Figure 1
Figure 1
(a) Effect of SR-27,897 (0.3 mg kg−1) on insulin plasmatic concentration. Vehicle (open columns) or SR-27,897 (hatched columns) were administered 120 min before the lights were off. Values are means±s.e.m. of 8 – 10 animals. (b) Effect of L-365,260 (1 mg kg−1) on insulin plasma concentration. Values are means± s.e.m. of 8 – 10 animals.
Figure 2
Figure 2
(a) Effect of SR-27,897 (0.3 mg kg−1) on leptin plasmatic concentration. Vehicle (open columns) or SR-27,897 (hatched columns) were administered 120 min before the lights were off. Values are means±s.e.m. of 8 – 10 animals. **P<0.01, compared to the control group (Newman – Keuls' test). (b) Effect of L-365,260 (1 mg kg−1) on leptin plasma concentration. Values are means± s.e.m. of 8 – 10 animals. *P<0.05, compared to the control group (Newman – Keuls' test).
Figure 3
Figure 3
Effect of SR-27,897 (0.3 mg kg−1) and L-365,260 (1 mg kg−1) on food intake. Values are means±s.e.m. of 8 – 10 animals. *P<0.05, compared to the control group (Newman – Keuls' test).
Figure 4
Figure 4
Effect of CCK-8 on the ratio between CSF and plasma leptin concentration. Values are means±s.e.m. of five to six animals. *P<0.05 (Newman – Keuls' test).
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References

    1. AKANA S.F., STRACK A.M., HANSON E.S., DALLMAN M. Regulation of activity in the hypothalamo – pituitary – adrenal axis is integral to a larger hypothalamic system that determines caloric flow. Endocrinology. 1994;135:1125–1134. - PubMed
    1. ATTOUB S., LEVASSEUR S., BUYSE M., GOIOT H., LAIGNEAU J.P., MOIZO L., HERVATIN F., LE MARCHAND-BRUSTEL Y., LEWIN J.M.M., BADO A. Physiological role of cholecystokinin B/gastrin receptor in leptin secretion. Endocrinology. 1999;140:4406–4410. - PubMed
    1. BADO A., LEVASSEUR S., ATTOUB S., KERMORGANT S., LAIGNEAU J.P., BORTOLUZZI M.N., MOIZO L., LEHY T., GUERRE-MILLOS M., LE MARCHAND-BRUSTEL Y., LEWIN M.J.M. The stomach is a source of leptin. Nature. 1998;394:790–793. - PubMed
    1. BARHAM D., TRINDER P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst. 1972;97:142–145. - PubMed
    1. BARRACHINA M.D., MARTÍNEZ V., WANG L., WEI J.Y., TACHÉ Y. Synergistic interaction between leptin and cholecystokinin to reduce short-term food intake in lean mice. Proc. Natl. Acad. Sci. U.S.A. 1997;94:10455–10460. - PMC - PubMed

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