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Review
. 2003 Oct;2(5):830-40.
doi: 10.1128/EC.2.5.830-840.2003.

trans and cis splicing in trypanosomatids: mechanism, factors, and regulation

Xue-hai Liang  1 , Asaf Haritan, Shai Uliel, Shulamit Michaeli
Affiliations
Review

trans and cis splicing in trypanosomatids: mechanism, factors, and regulation

Xue-hai Liang et al. Eukaryot Cell. 2003 Oct.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Mechanism of trans splicing. (A) Schematic representation of trans splicing. The 5′ splice site GU on the SL RNA and the 3′ splice site AG on the pre-mRNA are indicated. BP, branch point; Py, polypyrimidine tract. (B) Secondary structure of SL RNA. The three stem-loop structures (sl I, II, and III), the 5′ splice site, and the Sm-binding site are indicated. The shadowed dot at the 5′ end of SL RNA indicates the cap 4 structure.
FIG. 2.
FIG. 2.
Trypanosome snRNA repertoire. The snRNAs are organized around a cis-splicing substrate that can potentially bind all of the depicted trypanosome snRNAs. On the pre-mRNA, the 5′ and 3′ splice sites and polypyrimidine track (Py) are indicated. The trypanosome snRNAs are circled. The mammalian snRNA counterparts are boxed (30). The missing domains from the trypanosome snRNAs are in shaded boxes placed on the mammalian counterparts. The Sm-binding site is indicated by a black heavy line. Putative splicing factors are indicated.
FIG. 3.
FIG. 3.
Intra- and intermolecular base pairing across the SL RNA 5′ splice site. (A) Potential base-pairing interaction between U5-SL RNA and SLA1-SL RNA. The intramolecular base-pair interactions across the T. brucei 5′ splice site are indicated by thin bars. The interaction between the SL RNA and SLA1 is indicated by heavy bars, and the interactions with U5 are indicated by arrows (22). The cross-linked sites on SL RNA to either U5 or SLA1 are indicated by filled arrows (22, 102), and the cross-linked sites of SL RNA on SLA1 is indicated by an open arrow (102). The 5′ splice site is indicated by a gray arrow. (B) Potential for base-pair interactions of U5 with 5′ splice site of SL RNA from different trypanosomatid species. The nucleotides on SL and U5 snRNAs are numbered. The 5′ splice site is indicated by an open arrow. The most conserved interactions are boxed. Accession numbers (where available) and references for the SL and U5 snRNAs are as follows: T. brucei SL RNA (58) and the U5 RNA (22); L. collosoma SL RNA (58) and the U5 RNA (107); L. seymouri SL RNA X07487 and the U5 RNA (5); and C. fasciculata SL RNA J03470 and the U5 RNA AF182356.
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References

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