[A simple and highly efficient Agrobacterium-mediated rice transformation system]
- PMID: 14574993
[A simple and highly efficient Agrobacterium-mediated rice transformation system]
Abstract
A simple and highly efficient rice transformation system was established based on the studying of factors influencing the Agrobacterium-mediated rice transformation. Embryogenic calli derived from mature embryos were infected and cocultivated with A. tumefaciens EHA101 harboring binary vectors: pHQ9, pHQ10, pHQT3. The highest transformation frequency was about 100 hygromycin resistance calli per gram of calli explants, and above 85% of these calli could be regenerated into plants. The putative transgenic plants were confirmed by GUS assay and southern-blot analysis. Hygromycin resistance tests indicated that the segregation of transgene in T1 progeny corresponded to the Mendelian ratio, 3:1. This system will benefit the functional genomic study of rice by using T-DNA insertion mutagenesis and gene targeting.
Similar articles
-
Agrobacterium-mediated transformation of Javanica rice cv. Rojolele.Biosci Biotechnol Biochem. 2004 Jun;68(6):1193-200. doi: 10.1271/bbb.68.1193. Biosci Biotechnol Biochem. 2004. PMID: 15215580
-
Agrobacterium-mediated transformation of polyploid cereals. The efficiency of selection and transgene expression in wheat.Cell Mol Biol Lett. 2004;9(4B):903-17. Cell Mol Biol Lett. 2004. PMID: 15647806
-
Multigene Engineering in Rice Using High-Capacity Agrobacterium tumefaciens BIBAC Vectors.Methods Mol Biol. 2016;1385:29-37. doi: 10.1007/978-1-4939-3289-4_2. Methods Mol Biol. 2016. PMID: 26614279
-
Wheat (Triticum aestivum L.).Methods Mol Biol. 2006;343:245-53. doi: 10.1385/1-59745-130-4:245. Methods Mol Biol. 2006. PMID: 16988349 Review.
-
Transformation of rice mediated by Agrobacterium tumefaciens.Plant Mol Biol. 1997 Sep;35(1-2):205-18. Plant Mol Biol. 1997. PMID: 9291974 Review.
Cited by
-
Target Lines for in Planta Gene Stacking in Japonica Rice.Int J Mol Sci. 2022 Aug 20;23(16):9385. doi: 10.3390/ijms23169385. Int J Mol Sci. 2022. PMID: 36012650 Free PMC article.
-
Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System.Front Plant Sci. 2016 Mar 30;7:377. doi: 10.3389/fpls.2016.00377. eCollection 2016. Front Plant Sci. 2016. PMID: 27066031 Free PMC article.
-
Functional characterization of D-type cyclins involved in cell division in rice.BMC Plant Biol. 2024 Mar 1;24(1):157. doi: 10.1186/s12870-024-04828-9. BMC Plant Biol. 2024. PMID: 38424498 Free PMC article.
-
A base substitution in OsphyC disturbs its Interaction with OsphyB and affects flowering time and chlorophyll synthesis in rice.BMC Plant Biol. 2022 Dec 27;22(1):612. doi: 10.1186/s12870-022-04011-y. BMC Plant Biol. 2022. PMID: 36572865 Free PMC article.
-
A highly efficient genetic transformation system for broccoli and subcellular localization.Front Plant Sci. 2023 Mar 2;14:1091588. doi: 10.3389/fpls.2023.1091588. eCollection 2023. Front Plant Sci. 2023. PMID: 36937998 Free PMC article.