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. 1973 May;14(3):364-6.

Determination of cholesterol using o-phthalaldehyde

L L Rudel  1 , M D Morris
Affiliations
  • PMID: 14580182
Free article

Determination of cholesterol using o-phthalaldehyde

L L Rudel et al. J Lipid Res. 1973 May.
Free article

Abstract

A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl(3) method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl(3) determination (molar extinction coefficients of 11,610 and 33,440 for FeCl(3) and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.

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