Review
doi: 10.1016/s1367-5931(03)00097-8.
Mechanisms of protein fluorophore formation and engineering
Affiliations
- PMID: 14580558
- DOI: 10.1016/s1367-5931(03)00097-8
Item in Clipboard
Review
Mechanisms of protein fluorophore formation and engineering
Curr Opin Chem Biol.
2003 Oct.
Abstract
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-like proteins from Anthozoa species contain light-absorbing chromophores within their protein sequences. Recent studies have made progress in obtaining bright variants of these proteins that develop chromophores quickly and efficiently, as well as novel fluorescent proteins that photoconvert (i.e. change color upon illumination at specific wavelengths). Further molecular characterization of the structure and maturation of these proteins is in progress, aimed at providing information for rational design of variants with desired fluorescence properties.
Similar articles
-
Engineering fluorescent proteins.Adv Biochem Eng Biotechnol. 2005;95:1-15. doi: 10.1007/b102208. Adv Biochem Eng Biotechnol. 2005. PMID: 16080263 Review.
-
The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins.Nat Biotechnol. 2004 Mar;22(3):289-96. doi: 10.1038/nbt943. Nat Biotechnol. 2004. PMID: 14990950 Review.
-
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.Nat Biotechnol. 2002 Jan;20(1):87-90. doi: 10.1038/nbt0102-87. Nat Biotechnol. 2002. PMID: 11753368
-
Enhanced fluorescence resonance energy transfer between spectral variants of green fluorescent protein through zinc-site engineering.Biochemistry. 2001 Jan 30;40(4):938-45. doi: 10.1021/bi001765m. Biochemistry. 2001. PMID: 11170415
-
Fluorescent proteins as a toolkit for in vivo imaging.Trends Biotechnol. 2005 Dec;23(12):605-13. doi: 10.1016/j.tibtech.2005.10.005. Epub 2005 Nov 2. Trends Biotechnol. 2005. PMID: 16269193 Review.
Cited by
-
Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations.STAR Protoc. 2021 Feb 3;2(1):100315. doi: 10.1016/j.xpro.2021.100315. eCollection 2021 Mar 19. STAR Protoc. 2021. PMID: 33659898 Free PMC article.
-
A transgenic-cloned pig model expressing non-fluorescent modified Plum.J Reprod Dev. 2016 Oct 18;62(5):511-520. doi: 10.1262/jrd.2016-041. Epub 2016 Jul 11. J Reprod Dev. 2016. PMID: 27396383 Free PMC article.
-
Genetically encoded probes for measurement of intracellular calcium.Methods Cell Biol. 2010;99:153-82. doi: 10.1016/B978-0-12-374841-6.00006-2. Methods Cell Biol. 2010. PMID: 21035686 Free PMC article.
-
An improved short-lived fluorescent protein transcriptional reporter for Saccharomyces cerevisiae.Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 21. Yeast. 2012. PMID: 23172645 Free PMC article.
-
Plant protein-protein interaction network and interactome.Curr Genomics. 2010 Mar;11(1):40-6. doi: 10.2174/138920210790218016. Curr Genomics. 2010. PMID: 20808522 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
