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. 1982 Dec;2(12):1628-32.
doi: 10.1128/mcb.2.12.1628-1632.1982.

Expression of bacterial beta-galactosidase in animal cells

G An  1 , K Hidaka, L Siminovitch
Affiliations

Expression of bacterial beta-galactosidase in animal cells

G An et al. Mol Cell Biol. 1982 Dec.

Abstract

A recombinant plasmid containing the gene for bacterial beta-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

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References

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