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. 2003 Nov;22(4):261-7.
doi: 10.1007/s00299-003-0684-8. Epub 2003 Aug 29.

A novel selection system for potato transformation using a mutated AHAS gene

M Andersson  1 , A Trifonova, A-B Andersson, M Johansson, L Bülow, P Hofvander
Affiliations

A novel selection system for potato transformation using a mutated AHAS gene

M Andersson et al. Plant Cell Rep. 2003 Nov.

Abstract

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a beta-glucuronidase (GUS) gene. Selection on 0.5 microM Imazamox yielded GUS expression in 93-100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

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References

    1. Mol Gen Genet. 1991 Feb;225(2):289-96 - PubMed
    1. Theor Appl Genet. 1987 Sep;73(5):744-50 - PubMed
    1. Plant Mol Biol. 1985 Sep;5(5):299-302 - PubMed
    1. Plant Physiol. 1984 Jul;75(3):827-31 - PubMed
    1. Plant Mol Biol. 1992 Dec;20(6):1195-7 - PubMed

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