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. 1992 Nov;103(3):517-21.
doi: 10.1016/0305-0491(92)90363-v.

Purification and biochemical properties of calmodulin in Entamoeba histolytica and its distribution during secretion of electron-dense granules

M L Muńoz  1 , M S O'Shea-Alvarez, J Pérez-García, E C Weinbach, M A Moreno, M de la Torre, M A Magos, R Tovar
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Purification and biochemical properties of calmodulin in Entamoeba histolytica and its distribution during secretion of electron-dense granules

M L Muńoz et al. Comp Biochem Physiol B. 1992 Nov.

Abstract

1. Calmodulin (CaM) was detected during secretion of electron-dense granules by Entamoeba histolytica trophozoites with immunofluorescence. 2. It was purified to apparent homogeneity by chromatography with a yield of 2.26 micrograms of calmodulin/mg of protozoan protein. Purity was established by gel electrophoresis. 3. The parasite calmodulin has properties characteristic of calmodulin isolated from other eukaryotes: an apparent molecular weight of 19 or 17 kDa in presence of EGTA or CaCl2, respectively, activation in a calcium dependent manner of bovine heart cyclic nucleotide phosphodiesterase, and its UV spectrum.

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