doi: 10.1016/j.bmcl.2003.07.026.
An efficient enzymatic synthesis of thiamin pyrophosphate
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- PMID: 14592524
- DOI: 10.1016/j.bmcl.2003.07.026
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An efficient enzymatic synthesis of thiamin pyrophosphate
Bioorg Med Chem Lett.
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Abstract
Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.