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. 2003 Nov;56(11):844-9.
doi: 10.1136/jcp.56.11.844.

Evaluation of the IRIS 939 UDx flow microscope as a screening system for urinary tract infection

C Hughes  1 , M J Roebuck
Affiliations

Evaluation of the IRIS 939 UDx flow microscope as a screening system for urinary tract infection

C Hughes et al. J Clin Pathol. 2003 Nov.

Abstract

Aims: To evaluate the IRIS flow microscope for the examination of urine specimens and to compare its performance with that of manual methods; in addition, to assess its usefulness as a screening system for the detection of significant bacteriuria in a routine clinical laboratory.

Methods: The IRIS system was compared with manual quantitative microscopy for the detection of formed elements and with a variety of culture methods to detect urinary tract infection.

Results: The IRIS had a higher sensitivity for the detection of clinically significant formed elements than did manual methods, and using a combination of bacterial and white cell counts produced a sensitivity and specificity of 94.9% and 54.4%, respectively, for predicting positive significant growth, with a 98.5% predictive value for a negative result. Approximately 50% of specimens could be reported as negative on the day of receipt using the screening algorithm.

Conclusions: The sensitivity and specificity of the IRIS is equivalent to that of other automated screening systems and would remove the need for manual microscopy for most specimens. The system was easy to operate and is a useful addition to the methods currently available for the examination of urine specimens.

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Figures

Figure 1
Figure 1
The IRIS 939Udx instrument, optional strip reader, and view station.
Figure 2
Figure 2
Diagrammatic representation of hydrodynamic focusing.
Figure 3
Figure 3
Image analysis process of the IRIS 939 UDx.
Figure 4
Figure 4
An example of the “superscan” editing screen for online review.
See this image and copyright information in PMC

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