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. 2003 Oct;29(10):1996-2000.
doi: 10.1016/s0886-3350(03)00496-6.

Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis

Naoko Asano-Kato  1 , Ikuko Toda, Shigeto Shimmura, Tomoko Noda-Tsuruya, Kazumi Fukagawa, Masaru Yoshinaga, Akihiro Matsukawa, Kazuo Tsubota
Affiliations

Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis

Naoko Asano-Kato et al. J Cataract Refract Surg. 2003 Oct.

Abstract

Purpose: To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration.

Setting: Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.

Methods: To develop DLK in rabbit eyes, 25 microL of lipopolysaccharide (LPS) solution at a concentration of 50 microg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed.

Results: Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils.

Conclusions: The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.

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