Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Nov;41(11):5238-9.
doi: 10.1128/JCM.41.11.5238-5239.2003.

Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, gram stain-negative blood cultures

Hildegard Adler  1 , Nathalie Baumlin, Reno Frei
Affiliations

Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, gram stain-negative blood cultures

Hildegard Adler et al. J Clin Microbiol. 2003 Nov.

Abstract

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Harrell, L. J., S. Mirrett, and L. B. Reller. 1994. Subcultures of BACTEC-positive but gram or acridine orange stain-negative NR 6A and 7A blood culture bottles are unnecessary. Diagn. Microbiol. Infect. Dis. 20:121-125. - PubMed
    1. Hunter, J. S. 1993. Acridine orange staining as a replacement for subculturing of false-positive blood cultures with the BACTEC NR 660. J. Clin. Microbiol. 31:465-466. - PMC - PubMed
    1. Mirrett, S., R. J. Everts, and L. B. Reller. 2001. Controlled comparison of original vented aerobic FAN medium with new nonvented BacT/ALERT FA medium for culturing blood. J. Clin. Microbiol. 39:2098-2101. - PMC - PubMed
    1. Munson, E. L., D. J. Diekema, S. E. Beekmann, K. C. Chapin, and G. V. Doern. 2003. Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management. J. Clin. Microbiol. 41:495-497. - PMC - PubMed
    1. Snyder, J. W., K. S. Benzing, G. K. Munier, G. D. Bostic, P. S. Bozigar, R. Hanna, and M. J. Zervos. 2000. Clinical comparison of nonvented aerobic BacT/Alert blood culture bottle and standard aerobic bottle for detection of microorganisms in blood. J. Clin. Microbiol. 38:3864-3866. - PMC - PubMed

MeSH terms

LinkOut - more resources