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. 2003 Nov;87(11):1409-12.
doi: 10.1136/bjo.87.11.1409.

Decreased levels of cGMP in vitreous and subretinal fluid from eyes with retinal detachment

E C La Heij  1 , H G T Blaauwgeers, J de Vente, M Markerink, A T A Liem, A G H Kessels, H Steinbusch, F Hendrikse
Affiliations

Decreased levels of cGMP in vitreous and subretinal fluid from eyes with retinal detachment

E C La Heij et al. Br J Ophthalmol. 2003 Nov.

Abstract

Background: Cyclic guanosine monophosphate (cGMP) is produced in different retinal cells, including photoreceptor cells, wherein cGMP mediates photo-transduction. CGMP is degraded by phosphodiesterases (PDE). The aim was to investigate whether retinal detachment alters intraocular cGMP levels in human eyes.

Methods: cGMP and PDE were determined in vitreous fluid from 50 eyes with a retinal detachment (group I) and in 20 control samples (group II) of vitreous fluid from eyes without retinal detachment. Group III consisted of subretinal fluid samples from 70 eyes with retinal detachment.

Results: cGMP in vitreous fluid from eyes with retinal detachment (6.5 (SD 1.7) nM) was decreased compared to controls (67.1 (10.0) nM) (p<0.0001). In subretinal fluid, the mean level of cGMP was 2.4 (0.2) nM. No PDE could be detected in any of the intraocular fluid samples of patients nor controls. A decrease in the mean level of cGMP in subretinal fluid of eyes with retinal detachment correlated with a longer duration of detachment (r = -0.45, p = 0.007).

Conclusions: Retinal detachment was found to be associated with a decrease in vitreous cGMP concentration. In subretinal fluid, a low cGMP level correlated inversely with the duration of the detachment.

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Figures

Figure 1
Figure 1
cGMP levels (nM) in vitreous fluid from 50 eyes with retinal detachment (RD) (group I) compared to vitreous cGMP levels in 20 controls without retinal detachment (group II). (p<0.0001). A third group consisted of subretinal fluid from 70 eyes with retinal detachment (RD) (group III). The horizontal line represents the mean level in each group.
Figure 2
Figure 2
In patient group III, subretinal cGMP levels (nM) correlated moderately with the duration of the retinal detachment. The horizontal line represents the mean level in each group. A decrease in the mean level of cGMP was found if the retinal detachment existed longer. (p<0.0001. r = −0.45).
See this image and copyright information in PMC

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