doi: 10.1126/science.146257.
Membrane enzymes: artifacts in Arrhenius plots due to temperature dependence of substrate-binding affinity
- PMID: 146257
- DOI: 10.1126/science.146257
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Membrane enzymes: artifacts in Arrhenius plots due to temperature dependence of substrate-binding affinity
Science.
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Abstract
For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.
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