Comparative Study
doi: 10.1023/a:1026050627117.
Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures
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- PMID: 14626416
- DOI: 10.1023/a:1026050627117
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Comparative Study
Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures
Biotechnol Lett.
2003 Oct.
Abstract
The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
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