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. 2003 Dec;71(12):7170-2.
doi: 10.1128/IAI.71.12.7170-7172.2003.

Deletion of Mycobacterium tuberculosis sigma factor E results in delayed time to death with bacterial persistence in the lungs of aerosol-infected mice

Masaru Ando  1 , Tetsuyuki Yoshimatsu, Chiew Ko, Paul J Converse, William R Bishai
Affiliations

Deletion of Mycobacterium tuberculosis sigma factor E results in delayed time to death with bacterial persistence in the lungs of aerosol-infected mice

Masaru Ando et al. Infect Immun. 2003 Dec.

Abstract

The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a significant reduction of bacterial numbers in lungs.

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Figures

FIG. 1.
FIG. 1.
Analysis of CFU of wild-type, sigE-deleted, and sigE-complemented M. tuberculosis CDC1551 in lungs of C3H/HeJ mice after aerosol infection.
FIG. 2.
FIG. 2.
Analysis of time to death after aerosol infection with wild-type, sigE-deleted, and sigE-complemented M. tuberculosis CDC1551. Immunocompetent C3H/HeJ mice (A) showed a delayed time to death after infection with the knockout mutant (SigE-KO) compared to infection with the wild type (WT). BALB/c mice with the SCID mutation (B) succumbed by day 39 or 47 after infection with wild-type or sigE-complemented bacilli, respectively.
See this image and copyright information in PMC

References

    1. Brandt, L., J. Feino Cunha, A. W. Olsen, B. Chilima, P. Hirsch, R. Appelberg, and P. Andersen. 2002. Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis. Infect. Immun. 70:672-678. - PMC - PubMed
    1. Chen, P., R. E. Ruiz, Q. Li, R. F. Silver, and W. R. Bishai. 2000. Construction and characterization of a Mycobacterium tuberculosis mutant lacking the alternate sigma factor gene, sigF. Infect Immun. 68:5575-5580. - PMC - PubMed
    1. Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544. - PubMed
    1. Davis, N. K., and K. F. Chater. 1992. The Streptomyces coelicolor whiB gene encodes a small transcription factor-like protein dispensable for growth but essential for sporulation. Mol. Gen. Genet. 232:351-358. - PubMed
    1. DeMaio, J., Y. Zhang, C. Ko, D. B. Young, and W. R. Bishai. 1996. A stationary-phase stress-response sigma factor from Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 93:2790-2794. - PMC - PubMed

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