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. 2003 Dec;71(12):7223-7.
doi: 10.1128/IAI.71.12.7223-7227.2003.

Mycobacterium avium subsp. paratuberculosis infection causes suppression of RANTES, monocyte chemoattractant protein 1, and tumor necrosis factor alpha expression in peripheral blood of experimentally infected cattle

Joram J Buza  1 , Yasuyuki Mori, Abusaleh M Bari, Hirokazu Hikono, Aodon-geril, Sachiyo Hirayama, Yujing Shu, Eiichi Momotani
Affiliations

Mycobacterium avium subsp. paratuberculosis infection causes suppression of RANTES, monocyte chemoattractant protein 1, and tumor necrosis factor alpha expression in peripheral blood of experimentally infected cattle

Joram J Buza et al. Infect Immun. 2003 Dec.

Abstract

Blood from cattle with subclinical Mycobacterium avium subsp. paratuberculosis infection was stimulated with M. avium subsp. paratuberculosis antigens, and expression of interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), RANTES, monocyte chemoattractant protein 1 (MCP-1), and IL-8 was measured. Expression of TNF-alpha, RANTES, and MCP-1 was lower in infected than in uninfected cattle. The reduced response may weaken protective immunity and perpetuate infection.

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Figures

FIG. 1.
FIG. 1.
Kinetics of TNF-α in whole-blood samples from M. avium subsp. paratuberculosis-infected (•) and uninfected (○) cattle after in vitro stimulation with 0.5 μg of M. avium subsp. paratuberculosis PPD per ml (A), 10 μg of M. avium subsp. paratuberculosis lysate per ml (B), 60 × 106 live M. avium subsp. paratuberculosis CFU (C), 10 μg of E. coli LPS per ml (D), and medium (E). Each point represents the mean ± standard error of five animals. Differences between groups were considered significant when P < 0.05 (*).
FIG. 2.
FIG. 2.
Kinetics of RANTES in whole-blood samples from M. avium subsp. paratuberculosis-infected (•) and uninfected (○) cattle after in vitro stimulation with 0.5 μg of M. avium subsp. paratuberculosis PPD per ml (A), 10 μg of M. avium subsp. paratuberculosis lysate per ml (B), 60 × 106 live M. avium subsp. paratuberculosis CFU (C), 10 μg of E. coli LPS per ml (D), and medium (E). Each point represents the mean ± standard error of five animals. Differences between groups were considered significant when P < 0.05 (*).
FIG. 3.
FIG. 3.
Kinetics of MCP-1 in whole-blood samples from M. avium subsp. paratuberculosis-infected (•) and uninfected (○) cattle after in vitro stimulation with 0.5 μg of M. avium subsp. paratuberculosis PPD per ml (A), 10 μg of M. avium subsp. paratuberculosis lysate per ml (B), 60 × 106 live M. avium subsp. paratuberculosis CFU (C), 10 μg of E. coli LPS per ml (D), and medium (E). Each point represents the mean ± standard error of five animals. Differences between groups were considered significant when P < 0.05 (*).
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