Fast-MAS total through-bond correlation spectroscopy using adiabatic pulses

Abstract

Pulse sequences consisting of adiabatic pulses for total through-bond correlation spectroscopy (TOBSY) under magic-angle spinning (MAS) are introduced. Above a certain threshold, the polarization transfer achieved with these sequences is largely insensitive to the amplitude and homogeneity of the radiofrequency field employed. An experimental transfer efficiency of up to 76% was achieved in a two-spin system using the sequence WiW9(24)(1) at a MAS frequency of 26.67 kHz. Applications to resonance assignments in the dipeptide L-Val-L-Phe and in the cyclic decapeptide antamanide are demonstrated.