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. 2003 Dec;13(12):2754-8.
doi: 10.1101/gr.1499303.

A BAC-based physical map of the chicken genome

Chengwei Ren  1 , Mi-Kyung Lee, Bo Yan, Kejiao Ding, Bettye Cox, Michael N Romanov, Jennifer A Price, Jerry B Dodgson, Hong-Bin Zhang
Affiliations

A BAC-based physical map of the chicken genome

Chengwei Ren et al. Genome Res. 2003 Dec.

Abstract

A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.

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Figures

Figure 1
Figure 1
Example of a chicken BAC contig anchored to the chromosome 1 genetic map of the chicken genome. This contig consists of 142 clones from three source BAC libraries, contains 903 unique fingerprint bands, and is estimated to span 4.01 Mb. The clones prefixed with “h” were from the HindIII BAC library, with “b” from the BamHI BAC library, and with “r” from the EcoRI BAC library. The contig was anchored to the region around 361 cM of the chromosome 1 genetic map using five DNA markers, MSU0301, ADL0037, GCT0013, GCT0033, and ROS0081 (Groenen et al. 2000). The highlighted clones indicate the positive clones identified by DNA marker hybridization.
Figure 2
Figure 2
Example of a consensus band map (A) and the clone order fingerprints (B) of a BAC contig of the chicken physical map. These two maps are used to verify contig assemblies. The figure shows only part of the maps.
See this image and copyright information in PMC

References

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WEB SITE REFERENCES

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