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. 2003 Dec;69(12):7563-6.
doi: 10.1128/AEM.69.12.7563-7566.2003.

Genetic characterization of the carotenoid biosynthetic pathway in Methylobacterium extorquens AM1 and isolation of a colorless mutant

Stephen J Van Dien  1 , Christopher J Marx, Brooke N O'Brien, Mary E Lidstrom
Affiliations

Genetic characterization of the carotenoid biosynthetic pathway in Methylobacterium extorquens AM1 and isolation of a colorless mutant

Stephen J Van Dien et al. Appl Environ Microbiol. 2003 Dec.

Abstract

Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified. A colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. Mutations in the other three did not affect color. The tetracycline marker was removed from the original transposon mutant, resulting in a pigment-free strain with wild-type growth properties useful as a tool for future experiments.

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Figures

FIG. 1.
FIG. 1.
Summary of bacterial carotenoid biosynthesis pathways and associated genes compiled from references , , , , and .
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